CRUSTY: A Versatile Web Platform for the Rapid Analysis and Visualization of High-Dimensional Flow Cytometry Data

pubmed.ncbi.nlm.nih.gov

Flow cytometry (FCM) can investigate dozens of parameters from millions of cells and hundreds of specimens in a short time and at a reasonable cost, but the amount of data that is generated is considerable. Computational approaches are useful to identify novel subpopulations and molecular biomarkers …

Elucidate Neuro-Immune Interactions via Flow Cytometry

www.selectscience.net

Irritable bowel syndrome (IBS) is estimated to affect up to 10% of the global population. However – despite its prevalence – there is still a limited understanding of the causes and mechanisms of IBS, as well as no current cure nor proven effective treatment. In this video, Dr. Hind Hussein, postdoctoral researcher at the Translational Research...

DNA-Barcoded Signal Amplification for Imaging Mass Cytometry Enables Sensitive and Highly Multiplexed Tissue Imaging

pubmed.ncbi.nlm.nih.gov

Imaging mass cytometry (IMC) is a highly multiplexed, antibody-based imaging method that captures heterogeneous spatial protein expression patterns at subcellular resolution. Here we report the extension of IMC to low-abundance markers through incorporation of the DNA-based signal amplification by exchange reaction, immuno...

A Magneto-Activated Nanoscale Cytometry Platform for Molecular Profiling of Small Extracellular Vesicles

www.nature.com

Exosomal PD-L1 (exoPD-L1) has recently received significant attention as a biomarker predicting immunotherapeutic responses involving the PD1/PD-L1 pathway. However, current technologies for exosomal analysis rely primarily on bulk measurements that...

From Colonial Clusters to Colonial Sheaths: Analysis of Microcystis Morphospecies in Mesocosm by Imaging Flow Cytometry

www.researchsquare.com

The alarming increase in the frequency of blooms of Microcystis in freshwater lakes and reservoirs occurs worldwide, with major implications for their ecosystem functioning and water quality. We applied FlowCAM-based imaging flow cytometry together with PCR...

Analyzing Flow Cytometry—Profiling Blood Samples of Pancreatic Ductal Adenocarcinoma Patients

www.mdpi.com

We investigated the immunological changes in the blood of pancreatic ductal adenocarcinoma patients treated with a single cycle of FOLFIRINOX chemotherapy combined with lipegfilgrastim. We compared the use of flow cytometry and targeted gene...

Mapping Human Natural Killer Cell Development in Pediatric Tonsil by Imaging Mass Cytometry and High-Resolution Microscopy

www.biorxiv.org

Natural killer (NK) cells develop from CD34+ progenitors in a stage-specific manner defined by changes in cell surface receptor expression and function. Secondary lymphoid tissues, including tonsil, are sites of human NK cell development. Here we present new...

This newsletter is published by Association Revenue Partners

*This publication does not represent the thoughts or opinions of JSJD Media and is intended as an aggregation of published news content only