Flow Cytometry for Estimating Plant Genome Size: Revisiting Assumptions, Sources of Variation, Reference Standards, and Best Practices

journals.ashs.org

Flow cytometry has been widely used to estimate relative and absolute genome sizes (DNA contents) of plants for more than 50 years. However, the accuracy of these estimates can vary widely...

Pan-Cancer γδ TCR Analysis Uncovers Clonotype Diversity and Prognostic Potential

www.sciencedirect.com

Gamma-delta T cells (γδ T cells) play a crucial role in both innate and adaptive immunity within tumors, yet their presence and prognostic value in cancer remain underexplored. This study presents...

COPAS VISION Large Particle (10-1500 µm) Flow Cytometry with Imaging

Sponsored

Automated analysis, dispensing and image capture of viable multicellular organisms, cell clusters, bead-like particles and other samples that are too large/fragile for traditional flow cytometers.

Using Spectral Flow Cytometry for CAR T-Cell Clinical Trials: Game Changing Technologies Enabling Novel Therapies

www.mdpi.com

Monitoring chimeric antigen redirected (CAR) T-cells post-infusion in clinical trials is a specialized application of flow cytometry. Unlike the CAR T-cell monitoring for individual patients conducted in clinical laboratories...

Detailed Cellular and Spatial Characterization of Chronic Lung Allograft Dysfunction Using Imaging Mass Cytometry

www.jhltonline.org

Long-term survival after lung transplantation remains limited by chronic lung allograft dysfunction (CLAD), with two main phenotypes: bronchiolitis obliterans syndrome (BOS) and restrictive allograft syndrome...

Amplicon Sequencing With Internal Standards Yields Accurate Picocyanobacteria Cell Abundances as Validated With Flow Cytometry

academic.oup.com

To understand ecosystem state and function, marine microbial ecologists seek measurements of organismal abundance and diversity at high taxonomic resolution. Conventional flow cytometry accurately...

Characterization and Spatial Distribution of Infiltrating Lymphocytes in Medullary, and Lymphocyte-Predominant Triple Negative Breast Cancers

www.nature.com

Medullary carcinoma of the breast (MedBC) is a rare histological type that accounts for less than 5% of all invasive breast cancers. Here, we performed an exploratory study aimed to determine whether imaging...

Advancing CAR T-Cell Therapy

www.news-medical.net

CAR T-cell therapy, a groundbreaking treatment for certain cancers, particularly hematological malignancies, has shown some success where traditional treatments have failed. However, its potential...

Analysis of the Leishmania Mexicana Promastigote Cell Cycle Using Imaging Flow Cytometry Provides New Insights Into Cell Cycle Flexibility and Events of Short Duration

journals.plos.org

Promastigote Leishmania mexicana have a complex cell division cycle characterised by the ordered replication of several single-copy organelles, a prolonged S phase and rapid G2 and cytokinesis...

Cell Sorting Based on Pulse Shapes From Angle Resolved Detection of Scattered Light

www.nature.com

Analyzing and sorting cells at high throughput rates is a cornerstone in modern bio-medical research. A key technology for this purpose is flow cytometry, in particular fluorescence-activated cell sorting...

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