www.nature.com

Advanced bioimage analysis tools are poised to disrupt the way in which microscopy images are acquired and analyzed. This Focus issue shares the hopes and opinions of experts on the near and distant future of image analysis. Imagine receiving a text during breakfast. You glance at your phone and see that the text is not from your mom, but is rather from your microscope, informing you of the status of your overnight acquisition.

Clonally Expanded PD-1-Expressing T Cells Are Enriched in Synovial Fluid of Juvenile Idiopathic Arthritis Patients

onlinelibrary.wiley.com

In this study, the investigators found that despite the heterogeneity in the composition of synovial CD4+ and CD8+ T cells, those characterized by PD-1 expression were clonally expanded tissue-resident memory (Trm)-like cells and displayed the highest proinflammatory capacity, suggesting their active contribution in sustaining chronic inflammation in situ.

Optical Filters for Cytometry Applications / UV to NIR

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A 3D Tool for Measuring Intracellular Lipid Droplets

www.drugtargetreview.com

Tomographic phase microscopy in flow cytometry showcases its ability to accurately measure intracellular lipid droplets. In this article from Drug Target Review, the groundbreaking utilisation of tomographic phase microscopy in flow cytometry is examined, showcasing its ability to accurately measure intracellular lipid droplets in three dimensions without the need for labelling.

Ultraviolet Light Scattering Scanning Flow Cytometry in the Characterization of Submicron Microparticles

onlinelibrary.wiley.com

The performance of the ultraviolet light scattering (UVLS) in the analysis of individual particles with flow cytometry is evaluated. The main advantage of the UVLS relates to the improvement of the analysis of submicron particles due to the strong dependence of the scattering efficiency on the wavelength of the incident light.

An Optimized PDMS Microfluidic Device for Ultra-Fast and High-Throughput Imaging Flow Cytometry

pubs.rsc.org

Imaging flow cytometry (IFC) is a powerful tool for cell detection and analysis due to its high throughput and compatibility in image acquisition. Optical time-stretch (OTS) imaging is considered as one of the most promising imaging techniques for IFC because it can realize cell imaging at a flow speed of around 60 m s−1.

Real-Time Fluorescence Imaging Flow Cytometry Enabled by Motion Deblurring and Deep Learning Algorithm

pubs.rsc.org

Fluorescence imaging flow cytometry (IFC) has been demonstrated as a crucial biomedical technique for analyzing specific cell subpopulations from heterogeneous cellular populations. However, the high-speed flow of fluorescence cells leads to motion blur in cell images, making it challenging to identify cell type from the raw images.

The Do’s & Don’ts of Flow Cytometry in the Clinic

blog.championsoncology.com

Clinical flow cytometry is now a standard tool used by clinical researchers in numerous fields, especially immuno-oncology. Consider these “Do’s & Don’ts” of clinical flow cytometry as you decide to use this tool for clinical research.

Development of Novel PE and APC Near-Infrared Tandem Fluorochromes for Use in Spectral Flow Cytometry

biotium.com

Scientists are currently moving rapidly from the use of polychromic flow cytometry, the preferred technology for 45 years, to spectral flow cytometry, which now represents the most significant change in the half-century since flow cytometry was developed.

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