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PACMan: A Software Package for Automated Single‐Cell Chlorophyll Fluorometry
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Microalgae, small photosynthetic unicells, are of great interest to ecology, ecotoxicology and biotechnology and there is a growing need to investigate the ability of cells to photosynthesize under variable conditions. Current strategies involve hand-operated pulse-amplitude-modulated (PAM) chlorophyll fluorimeters, which can provide detailed insights into the photophysiology of entire populations- or individual...
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Method for Identifying Microplastics
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Microplastics, from the beads that were once commonplace in cosmetics to the weathered and broken-down remnants of trash, are now ubiquitous in marine and inland waters around the world. To date, though, scientists have struggled to identify which plastics persist longest in the environment and measure their abundance, especially at the smaller end of the size range where they’re most likely to be...
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Guidelines for Establishing a Cytometry Laboratory
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onlinelibrary.wiley.com
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The purpose of this document is to provide guidance for establishing and maintaining growth and development of flow cytometry shared resource laboratories. While the best practices offered in this manuscript are not intended to be universal or exhaustive, they do outline key goals that should be prioritized to achieve operational excellence and meet the needs of the scientific community.
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Rapid Deformability Cytometry for Tissue Biopsies
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www.nature.com
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The physical phenotypes of malignant cells in tissue biopsies can be rapidly characterized at high throughput via deformability cytometry after singularizing the cells into a suspension by using a tissue grinder. To validate their approach, Guck and co-authors initially...
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