Direct Comparison of Mass Cytometry and Single-Cell RNA Sequencing of Human Peripheral Blood Mononuclear Cells

www.nature.com

Single-cell methods offer a high-resolution approach for characterizing cell populations. Many studies rely on single-cell transcriptomics to draw conclusions regarding cell state and behavior, with the underlying assumption that transcriptomic readouts...

Evaluation of Sort Recovery via Rmax

currentprotocols.onlinelibrary.wiley.com

Cell sorting is a vital tool in biological studies. Its principal role is to purify subpopulations of cells or particles from suspensions of complex biological samples, allowing further analysis. Typically, cell sorters take monodispersed particles in a sample and accelerate them...

TRBC1 in Flow Cytometry: Assay Development, Validation, and Reporting Considerations

onlinelibrary.wiley.com

T-cell neoplasms are a diagnostically challenging group of diseases that require review of clinical presentation along with results from laboratory studies including histology and flow cytometry. Like most malignancies, demonstration of...

COPAS VISION Large Particle (10-1500 µm) Flow Cytometry with Imaging

Sponsored

Automated analysis, dispensing and image capture of viable multicellular organisms, cell clusters, bead-like particles and other samples that are too large / fragile for traditional flow cytometers.

Repli-Seq Sample Preparation Using Cell Sorting with Cell-Permeant Dyes

currentprotocols.onlinelibrary.wiley.com

Replication timing is significantly correlated with gene expression and chromatin organization, changes dynamically during cell differentiation, and is altered in diseased states. Genome-wide analysis of replication...

A Simple Protocol to Establish a Conditionally Immortalized Mouse Podocyte Cell Line

www.nature.com

Podocytes are specialized terminally differentiated cells in the glomerulus that are the primary target cells in many glomerular diseases. However, the current podocyte cell lines suffer from prolonged in vitro differentiation and...

Convolutional Neuronal Network for Identifying Single-Cell-Platelet–Platelet-Aggregates in Human Whole Blood Using Imaging Flow

onlinelibrary.wiley.com

Blood is a heterogeneous fluid and performs various functions, such as oxygen transport, nutrient transport, and buffering of fluctuations in the acid–base balance. Blood is omnipresent in all organs. As a result, it can ensure the...

Performance of a Novel Eight-Color Flow Cytometry Panel for Measurable Residual Disease Assessment of Chronic Lymphocytic Leukemia

onlinelibrary.wiley.com

Chronic lymphocytic leukemia (CLL) is a neoplasm characterized by the monoclonal proliferation of mature B-cells within the peripheral blood (PB), bone marrow (BM), and secondary lymphoid tissues and is the most...

Fluorescent Characterization of Differentiated Myotubes Using Flow Cytometry

onlinelibrary.wiley.com

During embryonic development, several phases of cellular proliferation and differentiation lead to the fusing of mononucleated myoblasts to form multinucleated myofibers, in a process known as myogenesis. This process is facilitated through the...

In-Depth Organic Mass Cytometry Reveals Differential Contents of 3-Hydroxybutanoic Acid at the Single-Cell Level

www.nature.com

Comprehensive single-cell metabolic profiling is critical for revealing phenotypic heterogeneity and elucidating the molecular mechanisms underlying biological processes. However, single-cell metabolomics remains challenging because of...

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