A Workflow for the Enrichment, the Identification, and the Isolation of Non-Apoptotic Single Circulating Tumor Cells for RNA Sequencing Analysis

onlinelibrary.wiley.com

During the last decades, cancer treatment gradually developed toward targeted therapies providing several benefits to patients such as reduced therapy-associated side effects and prolonged survival. Nevertheless, acquired resistances of tumor cells limit the success of any therapy pushing oncology research toward the development of optimized therapy prediction methods. However...

Comparative Analysis of Feature-Based ML and CNN for Binucleated Erythroblast Quantification in Myelodysplastic Syndrome Patients Using Imaging Flow Cytometry Data

www.nature.com

Myelodysplastic syndrome (MDS) is characterized as a highly heterogeneous group of clonal stem cell disorders marked by a range of different dysplastic changes in the bone marrow (BM) together with disrupted hematopoiesis, varying degrees of cytopenias, and an increased risk of progression to acute myeloid leukemia (AML). In the diagnostic setting, morphologic...

The Coulter Principle: A History

onlinelibrary.wiley.com

A historical description of Wallace H. Coulter's invention and development of his companies' instrumentation for analysis of blood cells or particles has never been available, and too often, this void has been addressed by conjectural or promotional publications. A history thesis, based on the author's access to Coulter's personal papers and to files kept by his father...

Massively Parallel In Vivo Perturb-Seq Reveals Cell-Type-Specific Transcriptional Networks in Cortical Development

www.sciencedirect.com

The authors report on the development of an AAV-based, massively parallel in vivo Perturb-seq platform to target broad tissues and cell types with gene-expression-based characterization at single-cell resolution. They report on their ability to profile...

CD Maps—Dynamic Profiling of CD1–CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets

www.frontiersin.org

Leukocytes display on their surface molecules that are crucial for sensing hazardous environmental changes and mediating cell adhesion and communication between cells both within the immune system and with stroma. These include...

Detection of Clonal Plasma Cells in POEMS Syndrome Using Multiparameter Flow Cytometry

www.nature.com

POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, monoclonal protein [M-protein], and skin changes) is a rare systemic disorder characterized by various symptoms that are a consequence of the presence of monoclonal...

MetaGate: Interactive Analysis of High-Dimensional Cytometry Data with Metadata Integration

www.sciencedirect.com

Fluorescence-based flow cytometry was invented in the late 1960s and has since gained widespread popularity in basic research, routine diagnostics, and clinical trials. Modern flow cytometers allow simultaneous quantification of...

On-Chip Flow Cytometer Using Integrated Photonics for the Detection of Human Leukocytes

www.nature.com

With the successful implementation of cell therapy in clinical practice, there is a need to increase the throughput and automation of cell analysis and isolation techniques. To guarantee the delivery of functional cells, cell therapies rely on the controlled processing of billions of cells by trained specialists in a limited timespan to ensure qualitative, cost-effective, and timely delivery to the...

Bulk Lysis Procedures Alter Target Cell Population Counts

onlinelibrary.wiley.com

The aim of this study was to evaluate bulk lysis protocols and compare them with minimal sample perturbation (MSP) protocols which are reported to better preserve the native cellular state and avoid significant cell loss due to washing steps. Applying bulk lysis protocols resulted in a significant decrease in non-necrotic cells compared to MSP protocols, suggesting...

Imaging Flow Cytometry of Tumoroids: A New Method for Studying GPCR Expression

onlinelibrary.wiley.com

Classically, confocal microscopy was used to visualize and quantify the expression of proteins such as G protein-coupled receptors (GPCRs) in cancer cells. As part of this approach, specific anti-GPCR antibodies were used to immunostain the cells, followed by the acquisition of images and computer analysis to determine co-localization between receptors and/or...

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