Performance of Spectral Flow Cytometry and Mass Cytometry for the Study of Innate Myeloid Cell Populations

pubmed.ncbi.nlm.nih.gov

Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations.

The International Society for Advancement of Cytometry (ISAC) to Share Research at the CYTO Annual Conference

www.einnews.com

The world’s top minds in the fields of cytometry, cell biology and immunotherapy will gather in Montréal, Canada for CYTO 2023, the 36th annual congress of the International Society for Advancement of Cytometry. Here we will share scientific discoveries and explore the latest developments in flow and image cytometry, microfluidics, advanced topics in data evaluation using artificial intelligence (AI), and other topics related to immunology, instrumentation, SRL management, fluorochrome development. ISAC’s annual conference is the focal point of the cytometry community and the 2023 theme

Webinar: Go With the Flow: Advancing Cell and Gene Therapy With Flow Cytometry

www.science.org

In 2017, 64 years after the publication of the structure of DNA, the first gene therapy became clinically available in the United States, a treatment for a sight-robbing eye disease. A year later, chimeric...

Comprehensive Quantitative Analysis of Erythrocytes and Leukocytes Using Trace Volume of Human Blood Using Microfluidic-Image Cytometry and Machine Learning

www.researchsquare.com

A diagnostic test, based on microfluidic image cytometry and machine learning has been designed and applied for accurate classification of erythrocytes, leukocytes including 5-part differentiation...

Flow Cytometry of Synaptoneurosomes (FCS) Reveals Increased Ribosomal S6 and Calcineurin Proteins in Activated Medial Prefrontal Cortex to Nucleus Accumbens Synapses

www.jneurosci.org

Learning and behavior activate cue-specific patterns of sparsely distributed cells and synapses called ensembles that undergo memory-encoding engram alterations. While Fos is often used to label selectively activated cell bodies...

Application of Flow Cytometry and Measurement of Water Activity to Evaluate the Stability of Probiotic Products

www.researchsquare.com

The flow cytometry method (FCM) is a widely renown practice increasingly used to assess the microbial viability of the probiotic products. Also, water activity (aw) can be used to verify of viable cells in probiotic products...

In Vivo Flow Cytometry Reveals an Anti-metastatic Effect of Rujifang in Triple-Negative Breast Cancer

pubmed.ncbi.nlm.nih.gov

Breast cancer is the most common cancer, and triple-negative breast cancer (TNBC) has the highest metastasis and mortality rate among all breast cancer subtypes. Rujifang is a traditional Chinese medicine formula with many years...

Imaging Mass Cytometry-Driven Spatial Analysis and Biomarker Discovery in Hodgkin Lymphoma

www.biorxiv.org

Previous studies have identified TME features that are prognostic and predictive, however these studies did not consider the entirety of TME cellular ecosystems, including precisely defined immune cell subsets with opposing inflammatory and immune-suppressive effects, as a determinant for differential...

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